Activity

Histopathological results were revealed tubular dilatation, tubular necrosis, loss of brushy margins, cast formation, and apoptotic bodies in tubular epithelial cells in the BIR group. There were no histopathological findings except for the swelling of tubule epithelial cells and the accumulation of proteinous material in some tubule lumens in renal tissue of desloratadine-treated rats.Conclusions Experimental results suggested that desloratadine may be useful in the treatment of renal I/R injury.Two-part mixed effects models are often used for analyzing longitudinal data with many zeros. Typically, these models are formulated with binary and continuous components separately with random effects that are correlated between the two components. Researchers have developed maximum-likelihood and Bayesian approaches for fitting these models that often require using particular software packages or very specialized software. We propose an imputation approach that will allow practitioners to separately use standard linear and generalized linear mixed models to estimate the fixed effects for two-part mixed effects models with complex random effects structures. An approximation to the conditional distribution of positive measurements given an individual’s pattern of non-zero measurements is proposed that can be easily estimated and then imputed from. We show that for a wide range of parameter values, the imputation approach results in nearly unbiased estimation and can be implemented with standard software. We illustrate the proposed imputation approach for the analysis of longitudinal clinical trial data with many zeros.Poly(L-lactide) (PLLA) as one of the most well-known biodegradable polyesters, has been studied extensively for bone tissue engineering. If being properly programmed, scaffolds from PLLA can also be endowed with the capability of shape memory. However, several noted issues, e.g., mechanically brittleness, high glass transition temperature 96%) compared to the unmodified PLLA fiber counterpart. Moreover, the PLLA-PHBV (73) fibers also show enhanced osteogenesis-inducing ability in the mouse bone mesenchymal stem cells, even under non-osteoinductive conditions. Collectively, for the first time this study demonstrates the enhanced shape memory and osteogenesis capabilities of the electrospun PLLA-PHBV composite fibers, and the researched PLLA-PHBV (73) fiber system could be potentially applied as a multifunctional scaffolding material for applications in bone tissue repair and regeneration.Background Enthusiasm for the use of metal nanoparticles in human and veterinary medicine is high. Many articles describe the effects of metal nanoparticles on microbes in vitro, and a smaller number of articles describe effects on the immune system, which is the focus of this review. Methods Articles were retrieved by performing literature searches in Medline, of the National Institute of Medicine, as well as via Google Scholar. Results In vitro studies show that metal nanoparticles have antimicrobial effects. Procaspase activation Some metal nanoparticles augment innate host immune defenses, such as endogenous antimicrobial peptides, and nitric oxide. Metal nanoparticles may also function as vaccine adjuvants. Metal nanoparticles can migrate to locations distant from the site of administration, however, requiring careful monitoring for toxicity. Conclusions Metal nanoparticles show a great deal of potential as immunomodulators, as well as direct antimicrobial effects. Before metal particles can be adopted as therapies; however, more studies are needed to determine how nanoparticles migrate though the body and on possible adverse effects.Various therapeutic approaches, e.g. in case of trauma or cancer require the transplantation of autologous tissue. Depending on the size and the origin of the harvested tissue, these therapies can lead to iatrogenic complications and donor site morbidities. In the future, these side effects could be avoided by transplanting artificially generated tissue consisting of different cells types and matrix components derived from the host body. Tissue that is grown in the patient could be advantageous compared to the more simply structured in vitro grown alternatives. In order to overcome the limitations of graft vascularisation the arteriovenous (AV) loop technique has been established for different tissues in the last years and was adapted for lymphatic tissue engineering in the present study. We utilized the AV loop technique to grow human lymphatic vasculature in vivo in the RNU nude rat. A combination of human lymphatic endothelial cells (LEC) and bone marrow-derived mesenchymal stem cells was implanted in a fibrin matrix surrounding the AV loop. After 2 or 4 weeks of implantation the animals were perfused and the tissue was harvested. It could be demonstrated by immunohistochemistry for human LYVE1, human CD31 and murine podoplanin, that the implanted cells formed human lymphatic vasculature in the AV loop chamber. Beside development of murine podoplanin positive vasculature in the AV loop tissue, vasculature positive for human marker proteins developed in comparable numbers. This suggests that implanted LEC are able to improve the lymphatic vascularisation of the newly engineered tissue. Thus, we were able to establish an in vivo tissue engineering method to generate lymphatic vascularized soft tissue. An axially vascularized transplantable lymphatic vessel network was engineered without requiring advanced cell culture equipment, rendering the lymphatic AV loop highly suitable for applied regenerative medicine.Introduction. Connected systems transmitting vital parameters could well represent a tool to shorten postoperative hospital stay while providing continuous remote patient monitoring and potentially detect the onset of complications. Our aim was to analyze the functionality of a transcutaneous biosensing data collection patch in morbidly obese patients. Materials and Methods. An adhesive patch (The HealthPatch MD™) was applied to patients’ chests postoperatively. The patch was connected to a tablet via a bluetooth network to collect the heart rate, respiratory rate, skin temperature, and posture recognition data. The tablet conveyed data to a secure health data central server by means of a WiFi or 3G/4G transmission. Data were stored in a digital health platform to which health care professionals could connect. The evaluation focused on the volume, quality, and security of data transmission. A pilot phase involved 10 patients. Thirty-three additional patients undergoing bariatric surgery were included in the experimental phase.