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The global emergence of zoonotic viruses, including poxviruses, poses one of the greatest threats to human and animal health. Forty years after the eradication of smallpox, emerging zoonotic orthopoxviruses, such as monkeypox, cowpox, and vaccinia viruses continue to infect humans as well as wild and domestic animals. Currently, the geographical distribution of poxviruses in a broad range of hosts worldwide raises concerns regarding the possibility of outbreaks or viral dissemination to new geographical regions. Here, we review the global host ranges and current epidemiological understanding of zoonotic orthopoxviruses while focusing on orthopoxviruses with epidemic potential, including monkeypox, cowpox, and vaccinia viruses.Suicidal behavior is a severe problem among drug users. This study examines influential factors related to suicide attempts and analyzes possible mediators of the relationship between perceived social support and suicide attempts amongst Chinese drug users under compulsory institutional drug treatment. Taking perceived social support as the independent variable, we found that the relationship between suicide attempts and perceived social support is mediated by self-esteem as a protective factor and depression as a risk factor. Path analysis shows that self-esteem contributes relatively more to the indirect effects than depression does, accounting for 31.1% and 24.2% of the total effect, respectively. Generally speaking, the findings of this study point to an urgent need for addressing suicide attempts among Chinese drug users while treating self-esteem as the protective factor that deserves as substantial attention as depression receives.Recent research showed that the chaotic maps are considered as alternative methods for generating pseudo-random numbers, and various approaches have been proposed for the corresponding hardware implementations. In this work, an efficient hardware pseudo-random number generator (PRNG) is proposed, where the one-dimensional logistic map is optimised by using the perturbation operation which effectively reduces the degradation of digital chaos. By employing stochastic computing, a hardware PRNG is designed with relatively low hardware utilisation. The proposed hardware PRNG is implemented by using a Field Programmable Gate Array device. Results show that the chaotic map achieves good security performance by using the perturbation operations and the generated pseudo-random numbers pass the TestU01 test and the NIST SP 800-22 test. Most importantly, it also saves 89% of hardware resources compared to conventional approaches.The inhibition behavior of carbon nanotubes (CNTs) and Gum Arabic (GA) on the corrosion of 316L stainless steel in CNTs-water nanofluid under the effect of different temperatures was investigated by electrochemical methods and surface analysis techniques. Thereby, 316L stainless steel samples were exposed to CNTs-water nanofluid under temperatures of 22, 40, 60 and 80 °C. Two concentrations of the CNTs (0.1 and 1.0 wt.% CNTs) were homogenously dispersed in deionized water using the surfactant GA and tested using three corrosion tests conducted in series open circuit test, polarization resistance test, and potentiodynamic scans. These tests were also conducted on the same steel but in solutions of GA-deionized water only. Tests revealed that corrosion increases with temperature and concentration of the CNTs-water nanofluids, having the highest corrosion rate of 32.66 milli-mpy (milli-mil per year) for the 1.0 wt.% CNT nanofluid at 80 °C. In addition, SEM observations showed pits formation around areas of accumulated CNTs that added extra roughness to the steel sample. The activation energy analysis and optical surface observations have revealed that CNTs can desorb at higher temperatures, which makes the surface more vulnerable to corrosion attack.SARS-CoV-2 is highly pathogenic in humans and poses a great threat to public health worldwide. Clinical data shows a disturbed type I interferon (IFN) response during the virus infection. In this study, we discovered that the nucleocapsid (N) protein of SARS-CoV-2 plays an important role in the inhibition of interferon beta (IFN-β) production. N protein repressed IFN-β production induced by poly(IC) or upon Sendai virus (SeV) infection. We noted that N protein also suppressed IFN-β production, induced by several signaling molecules downstream of the retinoic acid-inducible gene I (RIG-I) pathway, which is the crucial pattern recognition receptor (PRR) responsible for identifying RNA viruses. Moreover, our data demonstrated that N protein interacted with the RIG-I protein through the DExD/H domain, which has ATPase activity and plays an important role in the binding of immunostimulatory RNAs. These results suggested that SARS-CoV-2 N protein suppresses the IFN-β response through targeting the initial step, potentially the cellular PRR-RNA-recognition step in the innate immune pathway. Therefore, we propose that the SARS-CoV-2 N protein represses IFN-β production by interfering with RIG-I.In a previous study, we reported on the development of a synthetic polymer conjugate of pirarubicin (THP) that was formed via an acid-labile hydrazone bond between the polymer and the THP. However, the synthetic polymer itself was non-biodegradable, which could lead to unexpected adverse effects. Human serum albumin (HSA), which has a high biocompatibility and good biodegradability, is also a potent carrier for delivering antitumor drugs. The objective of this study was to develop pH-sensitive HSA conjugates of THP (HSA-THP), and investigate the release of THP and the cytotoxicity under acidic conditions in vitro for further clinical development. HSA-THP was synthesized by conjugating maleimide hydrazone derivatives of THP with poly-thiolated HSA using 2-iminothiolane, via a thiol-maleimide coupling reaction. GW4869 in vivo We synthesized two types of HSA-THP that contained different amounts of THP (HSA-THP2 and HSA-THP4). Free THP was released from both of the HSA conjugates more rapidly at an acidic pH, and the rates of release for HSA-THP2 and HSA-THP4 were similar. Moreover, both HSA-THPs exhibited a higher cytotoxicity at acidic pH than at neutral pH, which is consistent with the effective liberation of free THP under acidic conditions. These findings suggest that these types of HSA-THPs are promising candidates for further development.